A guava easycyte ht 2laser flow cytometer millipore was used with excitation by the blue 488nm laser and the gain controls at their default settings yellow and green fluorescence or set to 9. Ion sphere particles quality assessment user bulletin 7. Ion pgm hi q view chef kits thermo fisher scientific. This guide is intended for the guava easycyte 5, 62l, 8, and 12 instruments. Interested users must schedule an appointment for training with the cytometry core to discuss experimental design and expected outcomes. Determinations of algal numbers using the guava flow cytometer. Guava is the combination of numerous contributors, with a team that ensures the apis are consistent, allowing it to continuously evolve. Jun, 2007 guava easycyte mini is a microsyringebased flow cytometer equipped with a 488 nm blue laser, forward scatter detection and three channels for fluorescence detection. Please refer to the appropriate package insert for specific instructions and limitations. Guava easycyte from guava technologies,the guava easycyte system is the first and only blue laser based multiparameter cytometer with builtin 96well microplate sampling with tube options. Flow cells for newer guava easycyte systems now include minstac tubing. Use manual viacount analysis under these circumstances. Its single sample loader offers reliable tube sampling. Millipore guava easycyte ht flow cytometer system available from the lab world group.
Clarification made to dilute barcoded library pools, if used. The best resource is to monitor the users group, blogs, and presentations. When using third party programs to analyze exported data, please be sure to read the disclaimers in a previous section of this document. Inside the guava easycyte 12ht and guava easycyte 12 systems see page 7 for specifications. We strive to update the contents of our website and tutorials as timely and as precisely as. Setup procedure for guava easycyte with two lasers important.
The guava easycyte 5 flow cytometer offers absolute cell counts and five parameters 3 fluorescent colors, 2 light scatter, from an industry standard blue laser. Use the following metrics together to evaluate a sample, but not as. Guava software modules show all relevant data and results immediately. Guava easycyte mini from guava technologies biocompare. They use novel microcapillary technology and they are compact, userfriendly, and powerful enough emd millipore afs analyzer feed systems find emd millipore afs analyzer feed systems forums, questions, troubleshooting, discussions at. The culmination of over a decade of flow cytometry expertise, these instruments consume less sample, generate less. The easycyte mini is a benchtop flow cytometry system by guava. The guava easycyte 5ht system provides absolute cell counts and offers other highdemand features, such as five parameters 3 fluorescent colors, 2 light scatter and has a blue laser for access to commonly used fluorescent dyes. Guava easycyte system users guide guava pulp and guava leather. The user of this ebook is prohibited to reuse, retain, copy, distribute or republish any contents or a part of contents of this ebook in any manner without written consent of the publisher. Ion sphere particles quality assessment for the ion proton. Guava easycyte system users guide free ebook download as pdf file. Optical layout for the easycyte 12 threelaser system.
Guava easycyte flow cytometers from luminex corporation. Innovative guava microcapillary technology in the guava muse cell analyzer provides miniaturized systems for quick cell counts, viability, and basic cell health analyses, or step up to the guava easycyte system, a versatile benchtop platform for additional, multidimensional cell health assessments. Be sure to update your torrent server to the latest available version of torrent suite software before using this kit. The guava easycyte ht doesnt require sheathing fluid, so you can. Annexin v is calciumdependent phospholipid binding protein with high affinity for ps, a membrane component normally localized to the internal face of the cell membrane.
The millipore guava easycyte ht flow cytometer is a flexible, compact and reliable high throughput benchtop system. Now you can screen plates of cells and compounds quickly and easily, without the hassle of manipulating multiple tub,biological,biology supply,biology supplies,biology product. In addition, it is equipped with a highthroughput sampler able to automatically measure samples directly from a 96well plate or via micro. The user of this ebook is prohibited to reuse, retain, copy, distribute or republish. Comparison and evaluation of seven different bench. The guava instruments are based on microcapillary flow.
Widespread use of flow cytometry for immunophenotyping in clinical veterinary medicine is limited by cost and requirement for considerable laboratory space, staff time, and expertise. As a result, guava easycyte flow cytometers are uniquely amenable to ondemand use in the laboratory environment and have helped many. Guava easycyte 5ht base system from luminex selectscience. G112936 guava technologies guava easycyte plus w2 flow cells. The friendly and intuitive guavasoft software platform guides you through setup. These simple fluidics enable a selfaligning flow cell that can be removed for cleaning by the user. Follow the software instructions if prompted to put required tubessolutions for instrument tray check. Ion pgm hi q view ot2 kit thermo fisher scientific. This flow cytometer system produces dependable data for a wide range of multicolor cellular and bead assays in a highly userfriendly fashion and can handle 96well plates and up to 10 tubes in a single load. This flow cytometer system produces dependable data for a wide range of multicolor cellular and bead assays in a highly user friendly fashion and can handle 96well plates and up to 10 tubes in a single load.
The instrument accommodates 96well plates and up to 10 tubes in a single load. How it works the guava easycyte systems use patented, microcapillary, laserbased technology capable of detecting mammalian and microbial cells and beads. Guava easycyte ht bg flow cytometer shared equipment. The guava easycyte ht system users guide provides detailed information on. Note for easycyte or easycyte ht 5, 62l, and 8 system owners. If the correct dilution factor was entered in the guava express pro software, you can determine the total number of recovered isps as follows. Guava easycyte flow cytometers from luminex corporation description youll quickly find that guava easycyte benchtop microcapillary flow cytometers are simpler and cheaper to operate and maintain than traditional sheath fluidbased instruments enabling ondemand use in any lab. Setup procedure for guava easycyte with a single laser.
Guava easycyte, guava easycyte plus system computer for acquisition purposes. The guava easycyte mini was introduced to the cytometry core facility in january of 2010 through a partnership agreement with dr. Please refer to the appropriate package insert for specific instructions and. The ion 540 kit ot2 is compatible with torrent suite software v5.
The kit contains easycheck beads and the easycheck diluent. Guava easycheck software module issues addressed backflush for easycyte systems was not working in easycheck 2. Guava easycyte from guava technologies biomedicine. Rapid, precise, and accurate counts of symbiodinium cells. The guava easycyte plus guava technologies, hayward, ca, us is the first, personal, benchtop flow cytometer designed to address these limitations. No laser alignment or sheath fluid required uses smaller sample volume and generates less. Guava easycyte ht bg system is a flow cytometer for performing flow cytometry experiments. Dweck fls frsc frsh dweck data introduction in the next of our series on far eastern plant we look at guava or psidium guajava in folk medicine, extracts of roots, bark, and leaves are used to treat gastroenteritis.
This pc controlled cell analyzer utilizes microsyringe fluidics technology in conjunction with a 488 nm blue diode laser, forward scatter, and photomultipliers with three filters yellow583 nm, red680 nm, and green525 nm for conducting fluorescence detection. I have a demonstration in a couple of days and im interested in researchers experiences. These simple fluidics enable a selfaligning flow cell that can be removed for cleaning by. Millipore guava ht with plate handler protocol for multiplex assays this protocol contains instruction for setting up a millipore guava ht system.
The m1 cellml shows the total number of recovered isps. The muse cell analyzer packs 3parameter analysis into a compact. An easy to use, simple, compact, robust instrument for your cell analysis needs sophisticated cell analysis doesnt have to be complicated or costly. With the incorporation of the microsyringe fluidics, the read more. Has anyone experience with the easycyte 5 flow cytometer from millipore. Guava is an open source, javabased library and contains many core libraries. Figure 1a illustrates the overall standard cure conformity among. Ion sphere particles quality assessment for the ion proton and ion s5 systems using the guava easycyte 5 benchtop flow cytometer publication number man0007496 revision b.
Millipore guava ht with plate handler protocol for. Load the ion chef system and clean the ion chef instrument topics reorganized for ease of use. Mitosox red is excitable by a 488nm laser and fluoresces maximally at 580 nm yellow fluorescence on the guava easycyte 8ht. Manual adjustment for gain on the laser exciting the reporter molecule was not possible for instruments a and f. Follow the software instructions if prompted to put required tubessolutions for. Consult the guava easycyte system user s guide, guava easycyte ht system user s guide or guava easycyte plus system user s guide for more details. This flow cytometer system produces dependable data for a wide range of multicolor cellular and bead assays. If you created a worklist already, click start worklist. Use our product documents resource to find product manuals, help guides, coq, msds and more. Guava easycyte system users guide cytotoxicity apoptosis.
Dweck fls frsc frsh dweck data introduction in the next of our series on far eastern plant we look at guava or psidium guajava in folk medicine, extracts of roots, bark, and leaves are used to treat gastroenteritis, vomiting, diarrhoea, dysentery, wounds, ulcers, toothache, coughs, sore throat. The manual loader holds two separate tubes, allowing the user to progress quickly from one sample to another, or to wash between samples following an. For fully automated sample loading from single tubes or a 96well plate, we offer the easycyte ht high throughput systems, with four available highpower lasers. Running the easycheck procedure for daily quality control qc and maintenance ensures you are. Summary of new features, changes and enhancements since guavasoft software v3. May 31, 2017 millipore guava easycyte ht flow cytometer system available from the lab world group. Millipore guava easycyte ht flow cytometer system youtube. Spectrometers shared equipment authority rice university. Inside the guava easycyte 8ht and easycyte 8 systems see page 2 for specs for easycyte 5, 6, 62l, 5ht, 6ht and 6ht2l visits. Guava easycyte mini is a microsyringebased flow cytometer equipped with a 488 nm blue laser, forward scatter detection and three channels for fluorescence detection.
For fully automated sample loading from single tubes or a 96well plate, we offer the easycyte ht high throughput. References to isp quality control user guides updated to the ion sphere quality control kit user guide pub. Guava easycyte mini college of veterinary medicine. Setup procedure for guava easycyte with two lasers. Find msds or sds, a coa, data sheets and more information. The aim of this study was to adapt the immunohistochemical. If you need technical support, you can contact us 24 hours a day by email, call 18777852323, or visit our customer center for more local help. Has anyone experience with millipores guava flow cytometer.