The antimicrobial efficiency along with larvicidal activity of the synthesized nanoparticles was carried out by observing the lifecycle of mosquito for 96 hours along with. To identify the larvicidal activities of silver nano particles synthesised with rhizophora mucronata r. Biosynthesis of silver nanoparticles from mangrove plant. The present study was carried out to evaluate the larvicidal activity of leaf extracts of ambrosia arborescens and greensynthesized silver nanoparticles agnps using aqueous extracts obtained from this plant against third instar larvae of ae.
Characterization and larvicidal activity of greensynthesized silver nanoparticles using cracker plant ruellia tuberosa leaf extract th international conference and exhibition on nanomedicine and pharmaceutical nanotechnology july 2425, 2017 rome, italy. Mosquito larvicidal activity of silver nanoparticles synthesised using actinobacterium, streptomyces sp. Mar 14, 2012 silver nanoparticles cause more damage to testicular cells than titanium dioxide nanoparticles, according to a recent study. Larvicidal activity of synthesized silver nanoparticles agnps using aqueous extract from eclipta prostrata have been utilized to control the mosquito 30. The term being new, but has been widely used for the development of more efficient technology. Green synthesis of silver nanoparticles using solanum mammosum. The parasite larvae were exposed to varying concentrations of aqueous extract of. Since the nineteenth century, silverbased compounds have been used in many antimicrobial applications. Kahrilas ga, wally lm, fredrick sj, hiskey m, prieto al, owens je. Brindha durairaj, santhoshkumar muthu, and priya shanthi.
The fourth instar larvae of filariasis vector, culex quinquefasciatus say and malaria vector, anopheles subpictus grassi diptera. Department of biochemistry, psg college of arts and science, coimbatore 641014, tamilnadu. Antimicrobial activity of silver nanoparticles synthesized by using medicinal plants n. The silver nanoparticles, synthesized with the leaf extract of eclipta prostrata, were studied for their larvicidal activity against culex quinquefasciatus and anopheles subpictus3. Microbial nanoparticles synthesis is a greener approach by which it interconnects the nanotechnology and microbial biotechnology.
Synthesis of silver nanoparticles using nelumbo nucifera leaf. Therefore, it becomes urgent to develop novel alternative tools for vector control. The present study was carried out to evaluate the larvicidal activity of leaf extracts of ambrosia arborescens and greensynthesized silver nanoparticles agnps using aqueous extracts obtained. The peculiar characteristic of silver ions and compounds is their toxic impact on microorganisms.
Introduction malaria is by far the most important insect transmitted disease gilles and warrell, 1993, remaining a major health problem in many parts. Oct 27, 2010 read synthesis of silver nanoparticles using nelumbo nucifera leaf extract and its larvicidal activity against malaria and filariasis vectors, parasitology research on deepdyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips. In this study, we investigated the larvicidal activity of curcuma zedoaria essential oil zeo and biosynthesized silver nanoparticles using this essential oil zeoagnps. Mosquito larvicidal and antimicrobial activity of synthesized nanocrystalline silver particles using leaves and green berry extract of solanum nigrum l. Mosquito larvicidal activity of green silver nanoparticle synthesized. Oct 27, 2010 the aim of this study was to investigate the larvicidal potential of the hexane, chloroform, ethyl acetate, acetone, methanol, and aqueous leaf extracts of nelumbo nucifera gaertn. A total of 20 reared mosquito larvae was placed in 200 ml of double distilled sterilized water containing various concentrations 20. Antilarvicidal activity of silver nanoparticles synthesized from. It has long, bright green leaves clustered at the base of. Mosquito larvicidal activity of green silver nanoparticle synthesized from extract 19 which means fragrant at night. The present study was carried out to establish the pediculocidal and larvicidal activity of synthesized silver. Silver nanoparticles possess unique properties which find myriad applications such as antimicrobial, anticancer, larvicidal, catalytic, and wound healing activities. The larvicidal activity against both insecticidesusceptible.
Aug 30, 2018 the synthesized nanoparticles exhibited significant larvicidal activity. Abstract objective to identify the larvicidal activities of silver nano particles synthesised with rhizophora mucronata r. Mosquito control by silver nano particles 17 the larvicidal activity of silver nanoparticles against larvae of an. Larvicidal efficacy of silver nanoparticles synthesized. Suvarnalatha devi2 1department of botany, sri venkateswara university, tirupati 517 502, a. In agnps, the conduction band and valence band lie very close to each other in which electrons move freely. Larvicidal property of green synthesized silver nanoparticles against. Results atomic microscopic observation showed that the particle. The larvicidal activity against both insecticidesusceptible and resistant strains of cx. Nymphaeaceae and synthesized silver nanoparticles using aqueous leaf extract against fourth instar larvae of anopheles subpictus grassi and culex quinquefasciatus say diptera. Lab synthesized 20 nm stable silver nanoparticles were.
Page 2 declaration i tamasa panigrahi hereby declare that, this project report entitled synthesis and characterization of silver nanoparticles using leaf extract of azadirachta. The silver nanoparticles have various and important applications. The ag nano particles are spherical in shape with a smooth surface morphology. Larvicidal activity of silver nanoparticles synthesized using extracts of ambrosia arborescens asteraceae to control aedes aegypti l. In the present study activity of silver nanoparticles agnps synthesized using plumeria rubra plant latex against second and fourth larval instar of aedes aegypti and anopheles stephensi was determined. In addition, nanoparticles have been reported to have antiinflammatory 6, antiangiogenesis 7, antiviral 8 and antiplatelet activity. The mosquito species aedes aegypti is the primary vector of dengue, chikungunya, and zika infections worldwide.
Since effective vaccines or drugs are not available for the prevention andor treatment of these pathologies, vector control has been adopted as the main approach to reduce their transmission. Extraction and characterization of silver nano particles. Experimental antimicrobial effects of silver nanoparticles. The larvicidal activity was assessed by the procedure of who guidelines10 with some modification and as per the method of rahuman et al11. Nowadays, silver is extensively used as silver nanoparticles since its having large surface area than silver. Authors concluded that the antibacterial activity of silver nano particles is a beneficial application in crop improvement and protection in agricultural nanotechnology. An autolyzed cell free culture filtrate of selected isolate was used for the synthesis of silver nano particles and characterized using uv, ftir, xrd and afm analysis. Larvicidal activity of aqueous extracts and silver nanoparticles. Biosynthesis of silver nanoparticles and its applications. It has been noted that the introduction of silver nano particles has shown to have synergistic activity with common antibiotics already used today, such as. The present study was conducted to evaluate the larvicidal efficacies of agnps synthesized using aqueous leaf extracts of excoecaria agallocha against dengue vector, aedes aegypti. Stable silver nanoparticles were synthesized by biological reduction method. Patil college of pharmacy, akurdi, pune411044, maharashtra, india.
The biomolecules from various plant components and microbial species have been used as potential agents for the synthesis of silver nanoparticles agnps. Rawani a, ghosh a, gouta m c handra, mosquito larvicidal and antimicrobial activity of synthesized nanocrystallin e silver particles using leaves and gr een. A comparative study on larvicidal potential of selected. Larvicidal activity of synthesized silver nanoparticles. In that context, our study aimed at evaluating the insecticidal activity against ae. This method is considered as an innovative alternative approach using green nanochemistry technique to control vector parasites and is the first report on mosquito larvicidal activity of hibiscus vitifolius linn flowers mediated synthesized silver nanoparticles. The medical properties of silver have been known for over 2,000 years. Pdf biosynthesis of silver nanoparticles from morinda. The objective of the present study was to evaluate the larvicidal activity of silver nanoparticles synthesized from aqueous extracts of hibiscus vitifolius linn flowers against the larvae of aedes aegypti. Insecticide resistance and inadequate attention to the application instructions of topical pediculicides are common reasons for treatment failure.
In addition, because silver is an electron dense metal. Biosynthesis of silver nanoparticles by using mangrove. Antimicrobial and larvicidal activity of ecofriendly silver. Larvicidal activity of green synthesized silver nanoparticles using bark aqueous extract of ficus racemosa against culex quinquefasciatus and culex gelidus.
Find support for a specific problem on the support section of our website. Tem image also shows that the produced nano particles are more or less uniform in size and shape. Silver nanoparticles as a new generation of antimicrobials. The effect of silver nano particles on candida albicans and. Nov 18, 2011 thirunavukkarasu s, rahuman aa, govindasamy r, marimuthu s, asokan b, chidambaram j, zahir aa, elango g, chinnaperumal k 2010 synthesis of silver nanoparticles using nelumbo nucifera leaf extract and its larvicidal activity against malaria and filariasis vectors.
Polianthus tuberosa, silver nanoparticles, larvicidal activity, non target. Almond leaves for the onepot biofabrication of silver. Sagaya john paul j, rajkumar a, nivedha rajan n, photosynthesized silver nanoparticles from medicinal plant and their larvicidal activity against a edes aldopictus 2014, 174. Reports are available on larvicidal activity against various mosquitoes 19 20. Silver nanoparticles can be incorporated on many types of surfaces including metals, plastic, and glass.
Pdf larvicidal activity of silver nanoparticles synthesized using. Nanotechnology refers to the branch of science and engineering dedicated to materials, having dimensions in the order of 100th of nm or less. However, the problem arises when the procedure is over and a new one must be done. Research on nanoparticles opens up newer avenues for unraveling various biological challenges including the control of infectious diseases. Green synthesis of silver nanoparticles for the control of. In particular, the 200 nm silver particles caused a concentrationdependent increase in dna damage in the human cells. Characterization and larvicidal activity of greensynthesized. Larvicidal activity of silver nanoparticles synthesized using extracts. Larvicidal effects of nanosynthesized silver particles from. Jun 17, 2009 a tem image of the prepared silver nano particles is shown in the fig.
Insects free fulltext larvicidal activity of synthesized. Antimicrobial and larvicidal activity of ecofriendly. Effectiveness of silver nanoparticles of extracts of. Currently, silver sulfadiazine is listed as an essential antiinfective topical medicine by the world health organization who 2010. In medical equipment, it has been shown that silver nano particles lower the bacterial count on devices used compared to old techniques. Larvicidal activity of silver nanoparticles synthesized by. Introduction silver nanoparticles are particles of silver ranging in size from 1 to 100 nm. Biosynthesis of silver nanoparticles from leaf extract of. Larvicidal activity of synthesized silver nanoparticles using eclipta prostrata leaf extract against filariasis and malaria vectors. Screening of larvicidal activity of nanoparticles synthesized. In the present study an attempt was made to explore the larvicidal activity of silver nanoparticles synthesized using dried leaf extract of p.
Silver nanoparticles can prevent bacteria from growing on or adhering to the surface. Pdf on jan 1, 2014, ramesh kumar k and others published biosynthesis of silver nanoparticles from morinda tinctoria leaf extract and their larvicidal. Silver nanoparticles could also affect whole societies and ecosystems silver nanoparticles can inhibit many bacteria, including good bacteria silver nanoparticles can prevent photosynthesis in algae societal and ethical implications of nano silver. Gas chromatographymass spectrometry studies inferred the phytochemical constituents of the leaf extract. Nano facts nanotechnology is technology on the atomic and molecular scale. Larvicidal potential of fungi based silver nanoparticles. Larvicidal activity of synthesized silver nanoparticles from curcuma. Pandian2 1department of plant biology and plant biotechnology, presidency college, autonomous chennai, india 2university of madras, csir clri department of biotechnology, tamilnadu, india. Calotropis gigantea, silver nanoparticles, aedes aegypti, anopheles stephensi, larvicidal activity, green synthesis. Biogenic syntheses of silver nanoparticles using plants and their pharmacological and other potential applications are gaining momentum owing to its assured rewards.
Here, we report that ag nanoparticles can be applied effectively in the control of microorganisms and the prevention of deleterious infections. However, the use of both types may affect testicular cells with. Methods in vitro larvicidal activities such as lc50 and lc90 were assessed for the ae. Range of concentrations of synthesized agnps 10, 5, 2.
The present study focussed on the synthesis of agnp using p. Green synthesis of silver nanoparticles using solanum. In the present study, synthesis of silver nanoparticles using isoamyl acetate isolated from annona squamosa against mosquito larvae was carried out. The present study was carried out to evaluate the larvicidal activity of leaf extracts of. Santhoshkumar et al4 also synthesized silver nanoparticles using nelumbo nucifera leaf extract and studied their larvicidal activity against filariasis and malaria. Jocelyn l miranda, cherrie g muana and maria cleofe n badang. Mosquito larvicidal activity of green silver nanoparticle. The effect of silver nano particles on candida albicans. To control aedes populations, the most commonly used tool is the. Essential oils or plant extracts are good and safe alternatives due to their low toxicity to mammals and easy biodegradability.
Larvicidal activity of green synthesized silver nanoparticles. Authors concluded that the antibacterial activity of silver nanoparticles is a beneficial application in crop improvement and protection in agricultural nanotechnology. The elemental components demonstrated the appearance of silver without any contamination. The antimicrobial efficiency along with larvicidal activity of the synthesized nanoparticles was carried out by observing the lifecycle of mosquito for 96 hours along with appropriate controls. Pdf mosquito larvicidal activity of silver nanoparticles. In vitro larvicidal activity of agnps was used against the fourth instar of a. A number of reports have proved the efficacy of silver nanoparticles synthesized from bioresources is an effective antimicrobial agent, anticancer, photocatalytic, antioxidant and mosquitocidal ahmed et al. Larvicidal activity of synthesized silver nanoparticles from. Overdose of macro silver causes argyria safety of nano silver still unknown. Larvicidal property of green synthesized silver nanoparticles against vector mosquitoes anopheles stephensi and aedes aegypti. These free electrons give rise to a surface plasmon resonance spr absorption band, occurring due to the collective oscillation of electrons of silver nano particles in resonance with the light wave 79,80,81,82,83,84.
Synthesis of silver nanoparticles has the potential to be utilized as a good, ecofriendly approach for the control of mosquito population. The synthesized ag nps were characterized by uv, xrd, ftir and fesem. Extraction and characterization of silver nano particles synthesized using plant extract of kedrostis foeditissima jacq. It grows up to 45 cm 18 in long as elongated spikes that produce clusters of fragrant waxy white flowers. The lc50 and lc90 values of agnps showed to be effective. Larvicidal activity of silver nanoparticles synthesized using. The present investigation has been aimed to study larvicidal and antimicrobial activities of silver nanoparticles agnps synthesized using marine fluorescent pseudomonads. The biosynthesized silver nanoparticles augmented the larvicidal activity of c. Larvicidal activity of synthesized silver nanoparticles using isoamyl.
Green synthesis of silver nanoparticles agnps using plant extracts has been achieved by ecofriendly reducing and capping agents. This work proposes a novel synthetic scheme to produce a nanoparticle pesticide coreshell nano composite to be used as an active agent against insect vectors, such as mosquitoes. Log probit analysis and regression analysis of larvicidal activity of ag np of s. Pdf larvicidal activity of silver nanoparticles synthesized. Larvicidal activity of silver nanoparticles synthesized. Here, we report that ag nano particles can be applied effectively in the control of microorganisms and the prevention of deleterious infections. In the present study, an attempt has been made to identify the larvicidal activity of silver nanoparticles biosynthesised with a.
The diameter of the nano particles is found to be approximately 16 nm. Larvicidal activity of silver nanoparticles synthesized using extracts of ambrosia. The objective of this study was to produce silver nanoparticle from fresh bud of polianthes tuberosa and analyze the larvicidal activity against two important mosquito. M25 against anopheles subpictus, culex quinquefasciatus and aedes aegypti. The larvicidal and bitting detergency property of this plant against anopheles stephensi and cx. Biosynthesis of silver nanoparticles by using mangrove plant.
Synthesized silver nanoparticles agnps particles were. Pdf larvicidal property of green synthesized silver. After 12 hours of exposure, 50% mortality was observed and after 16 hours of treatment, 90% mortality was calculated in 1. Journal of pharmacology and toxicological studies larvicidal potential of fungi based silver nanoparticles against culex quinquefasciatus larvae ii and iii instar. The synthesized nano particles were subjected to larvicidal activity against larvae of anopheles stephensi following the method of rajakumar and rahuman, 2011. Nano bio pesticide to constraint plant destructive pests. The effect of silver nano particles on candida albicans and streptococcus mutans in denture acrylic resins. In this study, we investigated the larvicidal activity of curcuma zedoaria essential oil zeo and biosynthesized silver nanoparticles using this. In spite of a wide range of biomolecules assisting in the process, synthesizing stable and widely applicable agnps by many researchers still poses a considerable challenge to the researchers.